The Debug project, an initiative associated with Alphabet Inc., has petitioned the United States government for permission to deploy approximately 32 million modified mosquitoes annually across California and Florida to mitigate the transmission of vector-borne diseases.

Alphabet Inc. 旗下的 Debug 計劃已向美國政府申請許可，擬每年在加州與佛羅里達州投放約 3,200 萬隻經基因改造的蚊子，以降低病媒傳播疾病的風險。

The proposed intervention targets Aedes aegypti, an invasive African mosquito species responsible for the transmission of dengue, Zika, chikungunya, and yellow fever. The methodology utilizes Wolbachia, a naturally occurring bacterium. While the World Mosquito Program employs a 'replacement' strategy—releasing both sexes to establish a benign, non-transmitting population—the Debug project focuses on population suppression. By releasing exclusively Wolbachia-infected males, the program induces cytoplasmic incompatibility; mating between these males and wild females results in non-viable eggs, thereby precipitating a decline in the overall population density.

擬議的干預措施針對埃及 Aedes aegypti 蚊，這是一種入侵性的非洲蚊種，負責傳播登革熱、茲卡病毒、基孔肯亞熱和黃熱病。該方法利用一種天然存在的細菌 Wolbachia。雖然「世界蚊子計劃」採用的是「替代」策略——即投放雌雄兩性以建立一個溫和且不傳播疾病的族群——但 Debug 計劃則專注於族群抑制。透過僅投放感染 Wolbachia 的雄蚊，該計劃會誘導細胞質不親和現象；這些雄蚊與野生雌蚊交配後產生的卵無法孵化，從而導致整體族群密度下降。

Technological implementation is facilitated by proprietary sex-sorting machinery developed to isolate male specimens with high precision, as males do not engage in blood-feeding and thus pose no direct biting risk to humans. The efficacy of this approach was previously demonstrated in a 2018 CSIRO-backed study in Queensland, Australia, where populations in certain urban areas were reduced by over 80%.

技術實施由專有的性別分選設備協助，該設備能高精度地分離出雄性樣本，由於雄蚊不吸血，因此對人類不構成直接叮咬風險。此方法的功效此前已在 2018 年由澳洲 CSIRO 支持的昆士蘭研究中得到證明，當時部分城市地區的蚊子數量減少了 80% 以上。

Stakeholder positioning remains divided. Proponents, including some entomologists, argue that the invasive nature of Aedes aegypti minimizes the risk of cascading ecological disruptions, as the species lacks native predators in the target regions. Conversely, critics have expressed concerns regarding the scale of the biological intervention and the propriety of a corporate entity managing environmental modifications without broader public consensus. The project's operational structure has evolved from Verily Health, a life sciences entity, to direct acquisition by Google.

利益相關者的立場仍然分歧。支持者（包括部分昆蟲學家）認為，由於 Aedes aegypti 具有入侵性且在目標地區缺乏原生天敵，因此減少其數量將能將連鎖生態破壞的風險降至最低。相反，批評者對生物干預的規模，以及企業實體在缺乏廣泛公眾共識的情況下管理環境修改的適當性表示擔憂。該計劃的運作結構已從生命科學實體 Verily Health 演變為由 Google 直接收購。

The initiative currently awaits a decision from the US Environmental Protection Agency regarding the permits required to commence releases in California and Florida.

該計劃目前正等待美國環境保護署決定，以獲取在加州與佛羅里達州開始投放所需的許可證。